Sevoflurane induces ho-1-mrna expression by regulating P13K/Akt/P70S6K signaling pathway and affects neuronal apoptosis

Purpose: To determine the effect of sevoflurane (SE) on neuronal apoptosis, and mechanism involved.Methods: Sixty healthy male Sprague-Dawley rats were assigned to control, model SE groups. Sham surgery was performed in control group, while middle cerebral artery infarction (MCAO) established group. The expression HO-1 mRNA determined by quantitative real-time polymerase chain reaction (qRT-PCR). Apoptosis, autophagy protein content P13K/Akt/P70S6K signaling pathway assessed Western blot assay.Results: Apoptosis significantly lower rats, relative rats. There markedly higher levels LC3 II / I, beclin-1, bad, Bcl-2 caspase-3 groups than (p < 0.05). up-regulated groups, controls, but it phosphorylated proteins P13K/Akt /P70S6K signal-related up-regulated, elevated mice, when compared mice 0.05).Conclusion: improves behavior MCAO reduces infarction, inhibits apoptosis nerve cells regulating apoptosis-related through a that may be related induction HO-1-mRNA signal pathway. This provides new insights for development anti-neuronal drugs.